Safety data sheet northwest missouri state university. Methylene oxide, ac4553, ac4554, 41860, 41883 supplier. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or selfcontained breathing apparatus. We are happy to announce that we are fully compliant with ghs requirements domestically as well as internationally. Paraformaldehyde forms slowly in aqueous formaldehyde solutions as a. Inflammation of upper respiratory tract tissue was noted in these groups. Your new online msds binder is a place for you to store the material safety. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Is there a big difference between using formaldehyde or paraformaldehyde for cell fixation for immunofluorescence.
Electron microscopy sciences 157s paraformaldehyde methanol free solution. Formaldehyde and closely related formalin and glutaraldehyde is an organic chemical usually used in a water. However, we make no warranty of merchantability or any. Electron microscopy sciences 20% paraformaldehyde formaldehyde aqueous solution, 100 ml 20% paraformaldehyde aq solutn. Eye contact rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. This solution may be stored at room temperature for at least one year without change of the original quality. Working with formaldehyde could increase your chances of having fertility problems or miscarriage. Identification of the substancemixture and of the companyundertaking 1. The standard solution contains 4% paraformaldehyde in 0. Any organic m aterial will inactivate formaldehyde. Page 17 safety data sheet according to 19072006ec, article 31 printing date 19. Identification of the substancemixture and of the companyundertaking. Sigmaaldrich offers a number of paraformaldehyde products.
It also serves as a fixative in electron microscopy. It is also used in the preparation of formalin fixatives for tissues or cells. If it is necessary to purchase it in solid form, purchase prediluted or preweighed chemical preferably in granular or flake, rather than powder form in the least quantity needed to perform work. Formaldehyde was also present at concentrations of 3. It is used as an agricultural chemical, fungicide, bactericide, analytical reagent and wood preservative. Paraformaldehyde reagentgrade,crystalline 30525894. However i have come across a few methods and i just wanted to know if anyone has any suggestions on which method i should use. Formaldehyde, the decomposition product of paraformaldehyde, has been listed as a carcinogen by ntp and iarc.
Dow offers a broad line of glutaraldehydebased solutions, which are useful in a wide range of industrial and microbiocidal applications. They are produced and sold as aqueous solutions of glutaraldehyde, a linear, fivecarbon dialdehyde, containing up to 50 percent active by weight. Formaldehyde occupational safety and health administration. Paraformaldehyde is recommended for use in the preparation of formalin fixatives for tissues or cells when the samples are to be used in florescence studies. Formaldehyde, 20% vv aqueous solution msds msdsdigital. Paraformaldehyde is the most commonly used fixative employed for fixation of tissues and cells in immunohistochemistry and immunocytochemistry protocols. F indicates a substance that is the subject of a section 5f rule under tsca. Formaldehyde is a gas which when dissolved in water gives a formaldehyde solution. All containers of formaldehyde or paraformaldehyde must utilize tight fitting lids to prevent the release of formaldehyde. Weigh boats thermometer filter paper funnel 2 beakers wh2o big scooper graduated cylinder ph indicator strips scale hot and stirring plate 1 liter volumetric flask. If possible, do not work with paraformaldehyde in solid form. Paraformaldehyde solution 4% in pbs cas 30525894 scbt. Paraformaldehyde pfa is the smallest polyoxymethylene, the polymerization product of formaldehyde with a typical degree of polymerization of 8100 units. Formaldehyde formaldehyde is a colorless, strongsmelling gas often found in aqueous waterbased solutions.
H334 may cause allergy or asthma symptoms or breathing difficulties if inhaled. Tcl119 please refer disclaimer overleaf product description. To help prevent exposures, the preparation and use of formaldehyde or paraformaldehyde solutions are to utilize local exhaust ventilation systems such as a fume hood, slot hood, or articulating air exhaust system. Paraformaldehyde stock solution 16% add 8 g of paraformaldehyde to 50 ml of water. No component of this product present at levels greater than or equal to 0. Formalin is the name for saturated 37% formaldehyde solution. If swallowed and the victim is conscious, dilute, inactivate, or absorb th e ingested formaldehyde by giving milk, activated charcoal, or water. Incompatible with brass, steel, copper, acid anhydrides. If inhaled if breathed in, move person into fresh air. Add 1 n naoh dropwise while stirring until the solution clears.
Paraformaldehyde is the polymeric form of formaldehyde. P indicates a commenced pmn substance r indicates a substance that is the subject of a section 6 risk management rule under tsca. Paraformaldehyde is used as a fumigant, a disinfectant and in the manufacture of synthetic resins like melamine resin, phenol resin and polyacetal resin. Electron microscopy sciences 20% paraformaldehyde formaldehyde aqueous solution, 100 ml. Paraformaldehyde commonly has a slight odor of formaldehyde due to decomposition. Reasons for citation f paraformaldehyde is on the right to know hazardous substance list because it is cited by dot, nfpa and epa. View product specific information, msds, references and buying faq. Composition and information on ingredients composition. Other companies are charging thousands of dollars to set up accounts and give you access to their msds. Commonly used as a preservative in medical laboratories and mortuaries, formaldehyde is also found in many products such as chemicals, particle board, household products, glues, permanent press fabrics, paper product coatings, fiberboard, and plywood.
International inventories component tsca dsl ndsl einecs elincs nlp piccs encs aics china kecl. Material safety data sheet paraformaldehyde msds section 2. Paraformaldehyde is a white, crystalline sandlike solid with an odor of formaldehyde. Safety data sheet paraformaldehyde suppliers details. Hazard communication program for university of rochester. Paraformaldehyde solution 4% in pbs is commonly used to preserve tissues for routine histology in many labs. Is there a big difference between using formaldehyde or. Paraformaldehyde revision date 18jan2018 component casno weight % paraformaldehyde 30525894 90 4. Information on the written hazard communication program for formaldehyde in the workplace and how to readuse the information provided on sdss and labels. Formaldehyde, 37% ww safety data sheet according to federal register vol. Use precautions against formaldehyde exposure when opening containers or entering a poorly ventilated storage area. Formaldehyde, formalin, paraformaldehyde safe work.
Our msds database is an easy to use, do it yourself tool, and best of all. Show this safety data sheet to the doctor in attendance. Paraformaldehyde, 4% in pbs is used to preserve tissues for routine histology. Store in a cool, dry, wellventilated area away from incompatible substances.
Formaldehyde fixatives the microscopy alliance the. Commercially available formaldehyde solutions usually contain 1015% methanol, which prevents the formaldehyde from forming the polymeric paraformaldehyde. Paraformaldehyde revision date 18jan2018 polymer made with any freeradical initiator regardless of the amount used. The solution should be clear, colorless, with no precipitate and the ph 7. Paraformaldehyde 4% aqueous solution, em grade contd.
X listed e indicates a substance that is the subject of a section 5e consent order under tsca. One option is to purchase methanolfree formaldehyde aq in sealed ampoules. Exposure was stopped during the first week for the middle and high dose groups due to. The resource to the url or file is currently unavailable. No action shall be taken involving any personal risk or without suitable training. Formaldehyde, formalin, paraformaldehyde safe work practices.
If your lab is not a regular user of formaldehyde fixatives, there are a couple of easier options that we recommend. Exposure was stopped during the first week for the middle and high dose groups due to high mortality. It is changed to formaldehyde by heating and by adding small amount of sodium hydroxide. While paraformaldehyde has not been identified as a carcinogen, it should be handled with caution since formaldehyde t he monomer from which it is made is a carcinogen. Firstaid measures general advice if symptoms persist, call a physician.
Paraformaldehyde solution, 4% 4% paraformaldehyde in phosphate buffered saline product code. Here, you can learn more about formaldehyde and related chemicals and what you can do to reduce your exposure for a healthier pregnancy. Paraformaldehyde pfa solutions we make 16% pfa stock solution, and make 4% pfa for animal perfusion and slice fixation. A more efficient and rapid penetrant fixative used in combination with glutaraldehyde and acrolein fixatives. Do not handle until all safety precautions have been read and understood. Limited evidence of carcinogenicity in animal studies. Paraformaldehyde, formadehyde and formalin duke light. I need to make up 4% paraformaldehyde to fix some adherent cells.
Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Paraformaldehyde can be quite hazardous to handle and it is often difficult to get it to go into solution. Page 3 of 10 msds formaldehyde difficult, give oxygen. Paraformaldehyde extra pure msds cas 30525894 msds. In case of skin contact wash off with soap and plenty of water. Ce is a global chemical and specialty materials company that engineers and manufactures a wide variety of products essential to everyday living. Thus, a protocol calling for 10% formalin is roughly equivalent to 4% formaldehyde. Supplied as a 4% paraformaldehyde solution in 1x pbs. Beware though, that some solutions have methanol in them to stop polymerization but this could have a negative effect on your sample. Paraformaldehyde has been used for fixing of cells.
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